Journal: Cell reports

Article Title: Interferon-γ induces combined pyroptotic angiopathy and APOL1 expression in human kidney disease

doi: 10.1016/j.celrep.2024.114310

Figure Lengend Snippet:

Article Snippet: iPSC line BYS0110 (African American Male) , ATCC , ACS-1024.

Techniques: Plasmid Preparation, Recombinant, High Molecular Weight, Cell Culture, Derivative Assay, Modification, Software

Journal: Scientific reports

Article Title: Effect of extracellular vesicles derived from induced pluripotent stem cells on mesangial cells underwent a model of fibrosis in vitro.

doi: 10.1038/s41598-023-42912-6

Figure Lengend Snippet: Figure 1. EV-iPSCs characterization and internalization by MMCs. Histogram representing the profile of nanoparticle size by Nanoparticle Tracking Analysis (A). Western blot analysis for extracellular vesicle markers (B). Cytoskeleton is stained with phalloidin (green) (C). The nucleus is stained with DAPI (blue) (C). EV-iPSCs are stained with CMTPX (red) (C). Colocalization of all markers (C). The arrows show the incorporation of EV-iPSCs by MMCs. 20× magnification. Images are representative.

Article Snippet: Human induced pluripotent stem cell culture Human induced pluripotent stem cells (iPSCs) were purchased from ATCC (BYS0112) and cultured at 37 °C in 25 cm2 flasks using CellMatrix Basement Membrane Gel (ATCC ACS-3035) in Pluripotent Stem Cell SFM XF (ATCC ACS-3002) medium.

Techniques: Western Blot, Staining

Journal: Scientific reports

Article Title: Effect of extracellular vesicles derived from induced pluripotent stem cells on mesangial cells underwent a model of fibrosis in vitro.

doi: 10.1038/s41598-023-42912-6

Figure Lengend Snippet: Figure 2. Effect of EV-iPSCs on gene and protein expression of ECM components. The gene expression of fibronectin (A), vimentin (B), α-SMA (C), collagen I (D) and collagen IV (E) was evaluated by RT-PCR. The protein expression of vimentin (F) and α-SMA (G) was evaluated by western blotting. HPRT and β-actin were used as the reference gene and protein, respectively. The immunofluorescence of fibronectin was evaluated and quantified by microscopy (H). The fibronectin marker is observed in red, and the nucleus was stained with DAPI (blue). 40× magnification. Images are representative of independent experiments. The results are expressed as the mean ± SEM. p < 0.05: * versus group control; # versus group TGF-β.

Article Snippet: Human induced pluripotent stem cell culture Human induced pluripotent stem cells (iPSCs) were purchased from ATCC (BYS0112) and cultured at 37 °C in 25 cm2 flasks using CellMatrix Basement Membrane Gel (ATCC ACS-3035) in Pluripotent Stem Cell SFM XF (ATCC ACS-3002) medium.

Techniques: Expressing, Gene Expression, Reverse Transcription Polymerase Chain Reaction, Western Blot, Immunofluorescence, Microscopy, Marker, Staining, Control

Journal: Scientific reports

Article Title: Effect of extracellular vesicles derived from induced pluripotent stem cells on mesangial cells underwent a model of fibrosis in vitro.

doi: 10.1038/s41598-023-42912-6

Figure Lengend Snippet: Figure 3. Effect of EV-iPSCs on gene and protein expression of RAS components. The mRNA expression of renin (A), angiotensinogen (B), and AT1 receptor (C) was evaluated by RT-PCR. The protein expression of renin (D) and angiotensinogen (E) was evaluated by western blot. HPRT and β-actin were used as the reference gene and protein, respectively. The immunofluorescence of angiotensinogen and AT1 receptor was evaluated and quantified by microscopy (F–G). Angiotensinogen and AT1 receptor markers are observed in red, and the nucleus was stained with DAPI (blue). 40× magnification. Images are representative of independent experiments. The results are expressed as the mean ± SEM. p < 0.05: * versus group control; # versus group TGF- β.

Article Snippet: Human induced pluripotent stem cell culture Human induced pluripotent stem cells (iPSCs) were purchased from ATCC (BYS0112) and cultured at 37 °C in 25 cm2 flasks using CellMatrix Basement Membrane Gel (ATCC ACS-3035) in Pluripotent Stem Cell SFM XF (ATCC ACS-3002) medium.

Techniques: Expressing, Reverse Transcription Polymerase Chain Reaction, Western Blot, Immunofluorescence, Microscopy, Staining, Control

Journal: Scientific reports

Article Title: Effect of extracellular vesicles derived from induced pluripotent stem cells on mesangial cells underwent a model of fibrosis in vitro.

doi: 10.1038/s41598-023-42912-6

Figure Lengend Snippet: Figure 4. Effect of EV-iPSCs on gene and protein of NF-κB-mediated inflammatory and SMAD pathway. The gene expression of p50 (A), p65 (B), IKKα (C), SMAD-3 (G) and SMAD-7 (H) was evaluated by RT-PCR. The protein expression of p50 (D), p65 (E), and IKKβ (F) was evaluated by western blot. HPRT and β-actin were used as the reference gene and protein, respectively. The results are expressed as the mean ± SEM. p < 0.05: * versus group control; # versus group TGF-β.

Article Snippet: Human induced pluripotent stem cell culture Human induced pluripotent stem cells (iPSCs) were purchased from ATCC (BYS0112) and cultured at 37 °C in 25 cm2 flasks using CellMatrix Basement Membrane Gel (ATCC ACS-3035) in Pluripotent Stem Cell SFM XF (ATCC ACS-3002) medium.

Techniques: Gene Expression, Reverse Transcription Polymerase Chain Reaction, Expressing, Western Blot, Control